This is the section for methodologies on Nodulation and nodule physiology.
Submitted methodologies are listed below. As the resource centre expands, methodologies may be grouped. Click on a title to move lower down the page for a brief synopsis and links to files or external web pages.
- Note on isolation and DNA extraction of rhizobia
- Isolation of bacteria from root nodules
- Nodule bacteria DNA extraction and PCR
Note on isolation and DNA extraction of rhizobia
The following file gives an introduction to the Standard Operating Procedures (SOP) in the next two entries for the isolation and extraction of rhizobia from legumes and the extraction and analysis of microbial DNA
File for download" Word 16 kb): Note_on_isolation_and_DNA_extraction_of_rhizobia
Isolation of bacteria from root nodules
SOP Isolation of bacteria from root nodules (Word file 23 kb)
Bacterial DNA extraction and PCR
SOP Nodule bacteria DNA extraction and PCR (Word file 34 kb)
Author: Euan James, James Hutton Institute UK. First published on this site 25 August 2011.
The following protocol has now been replaced by the ones above: Isolation of rhizobia from legume nodules - NB no longer current
